Gateway cloning invitrogen manual

Gateway ® destination vector of choice to generate an expression clone which may then be used in the appropriate application or expression system. 4. Convert your own vector to a destination vector. For details about a particular Life Technologies destination vector or expression system, refer to the manual for the specific destination vector File Size: 1MB. containing expression clone. Store Gateway™ LR Clonase™ II enzyme mix at –20°C (non-frost-free freezer) for up to 6 months. For long-term storage, store at –80°C. Gateway™ technology Gateway™ Technology is a universal cloning method that takes advantage of the site-File Size: KB. Instruction Manual Gateway® pENTR™ Vectors Catalog nos. , , , , Version B 23 November A Limited Label License covers this product (see PurchaserFile Size: KB.

The easy-to-use choice for everyday cloning. Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. The Gateway® Technology is a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, ) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems. To express your gene of interest using the Gateway® Technology, simply: 1. containing expression clone. Store Gateway™ LR Clonase™ II enzyme mix at –20°C (non-frost-free freezer) for up to 6 months. For long-term storage, store at –80°C. Gateway™ technology Gateway™ Technology is a universal cloning method that takes advantage of the site-.

Clone your gene of interest into a Gateway® entry vector to create an entry clone. small-scale yeast transformation protocol is included in the. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway® cloning technology (Invitrogen Co.) has. Oct Making Entry Clones for 2-Fragment Recombination. protocol provided on page 34 to perform the BP recombination reaction using BP.